Research Articles
Optimized RNA Extraction from FFPE Samples for Reliable qPCR: A Complete Guide for Researchers
This article provides a comprehensive guide for researchers and drug development professionals on extracting high-quality RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissues for accurate qPCR analysis.
Advanced Real-Time PCR Protocols for Circulating Tumor DNA: From Foundational Principles to Clinical Applications in Precision Oncology
This comprehensive review details the pivotal role of real-time PCR technologies, particularly digital PCR (dPCR) and droplet digital PCR (ddPCR), in the analysis of circulating tumor DNA (ctDNA) for cancer...
RT-qPCR in Liquid Biopsy: A Comprehensive Guide for Cancer Detection and Monitoring
This article provides a detailed examination of Reverse Transcription Quantitative PCR (RT-qPCR) for liquid biopsy applications in oncology, tailored for researchers and drug development professionals.
Beyond GAPDH and ACTB: A Modern Guide to Selecting Stable Reference Genes for Accurate qPCR in Cancer Research
Accurate gene expression analysis via qPCR is foundational to cancer research, yet a pervasive reliance on traditional reference genes like GAPDH and ACTB frequently leads to data distortion and irreproducible...
MIQE Guidelines for Cancer Biomarker Validation: Ensuring Reproducibility in Molecular Diagnostics
This article provides a comprehensive guide for researchers and drug development professionals on implementing MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) guidelines specifically for cancer biomarker validation.
Real-Time PCR in Transcriptional Biomarker Discovery: A Foundational Guide from Discovery to Clinical Application
This article provides a comprehensive overview of the critical role real-time quantitative PCR (qPCR) plays in the discovery and validation of transcriptional biomarkers for drug development and clinical diagnostics.
Benchmarking 3D-QSAR Against Molecular Docking: A Practical Guide for Predictive Drug Discovery
This article provides a comprehensive framework for researchers and drug development professionals to benchmark 3D-QSAR models against molecular docking results.
Field-Based vs. Similarity-Based 3D-QSAR: A Comprehensive Guide for Modern Drug Discovery
This article provides a thorough comparative analysis of two foundational 3D-QSAR methodologies: field-based and similarity-based approaches.
2D-QSAR vs. 3D-QSAR: A Comprehensive Performance Comparison for Glioblastoma Drug Discovery
This article provides a detailed comparison of 2D and 3D Quantitative Structure-Activity Relationship (QSAR) models in the context of glioblastoma multiforme (GBM) therapeutics.
CoMFA vs. CoMSIA: A Comparative Analysis of Predictive Accuracy in Cancer Drug Design
This article provides a comprehensive comparison of two cornerstone 3D-QSAR techniques—Comparative Molecular Field Analysis (CoMFA) and Comparative Molecular Similarity Indices Analysis (CoMSIA)—in the context of cancer drug discovery.